LECTURES #7 AND #8
SUBCLASS POLYTRICHIDAE

(the hair-cap mosses)

 

Text: pages 57 - 73

 

- approximately 19 genera, and about 370 species

- one order Polytrichales, two families Polytrichaceae, and Dawsoniaceae (one genus, ~12 species)

- they grow well on acidic and nutrient-poor substrata, thus they can be abundant in open terrain

- Arctic to Antarctic from sea level to high alpine

 

Characteristics:

 

(a) spore

- unicellular when shed

- extremely small (50um in Dawsonia >65 mill spores in one sporangium)

 

(b) protonema

- spore germinates to produce a protonema

- protonema (chloronema and caulonema)

- protonema may coat soil and then disappear after gametophores are produced, some persist for many years (Pogonatum)

 

(c) Gametophyte

 

- the gametophore varies considerably in size.

- some gametophores are a few millimeters tall, while others may exceed a decimeter (Dawsonia  >60cm tall)

- still others, if one considers the accumulated growth of preceding years, can form dense turfs more than a meter deep (P. strictum).

 

- Most are dioicous (antheridial and archegonial plants are separate). 

- both sexes have an erect stem that is unbranched or little branched (some exceptions of course)

- the lower part of the stem is attached to the substratum by a network of rhizoids.

 

(d) Stem

- in cross-section, has stereid cells in the cortical area, many parenchyma cells, and often a central cylinder of hydroids that make up the hydrome, an internal conducting system,structurally and functionally resembling the xylem of vascular plants. 

- the hydrome consists of elongate dead cells.

- surrounding the hydrome is an often ill-defined layer of living cells that transport metabolites, this consists of leptoids, and constitutes the leptome. 

- this internal conducting system is often connected to the hydroids and leptoids of the leaves, and thus forms an effective, if rather inefficient, internal vascular system

- water uptake in the hair cap mosses is effected by the water held in capillary spaces among the rhizoids, enclosed by sheathing leaf bases, and spaces among the lamellae.

 

- water can, therefore, enter the chlorophyllose leaf cells directly, can enter the stem through the cortical cells, or can enter the interior portion of the stem from the part of the stem that is embedded in the soil. 

- water conduction up and down the stem is both external (ectohydric) and internal (endohydric) and is very slow.

- in subterranean parts of the stem you see the same conductive and supportive cells, but there is an interesting single layer of cells within the stereids which are enlarge.  It is speculated that they serve as an endodermis

 

(e) Leaves

- 6-8 leaves spirally arranged on the stem and divergent when wet and contorted or imbricate when dry

- usually long and lanceolate, sometimes toothed, often has an expanded colorless sheath, often abrupt "hinge" inward when dry

- usually have a costa, and on this midrib are often the lamellae on adaxial surface of leaf.

- lamellae and most of the leaf blade (except the midrib) are a single cell in thickness (unistratose). 

 

- What is the function of the lamellae?

- in some species of Polytrichum and Dawsonia the only photosynthetic cells are found in the lamellae

- the upper edge of the lamellae and often the undersurface  of the leaf, especially on the midrib are heavily cutinized. 

- increased phtosyntheitc surface area

- Atrichum has reduced lamellae, no sheathing base, entire blade is photosynthetic

- Alophosia - entire blade is bistratose

 

- the midrib of the leaf is usually reinforced by thick-walled cells, the stereids.  The supportive cells give the leaf rigidity and are also significant in water transport. 

- the midrib often has hydroid cells, and sometimes leptoids.

- there are some species with very reduced gametophore perigonium and perichaetium often protonema is perennial (Pseudoracelopus, Racelopus, Racelpodopsis)

 

(f) Reproduction

- asexual reproduction by gemmae only found in one genus (Alophosia) disc-like gemmae, subterranean fragmentation, can see Atrichum leaves in the soil with protonema sticking out

 

- the antheridial plant, when sexually mature, is terminated by a cluster of expanded leaves, usually with little chlorophyll, and often pigmented with brown or reddish colour.

- this cup-like mass of leaves forms the perigonium and encloses, at the axils of each of the leaves, clusters of filaments, the paraphyses, and antheridia.  The antheridia are elongate. In the centre of the perigonium an apical cell remains meristematic. 

- the paraphyses - multiseriate and club-shaped in some genera while in others uniseriate

- mucilage is produced (females too) which may attract insects to attract invertebrates for sperm dispersal

- the perigonium is thus shaped like a splash-cup, and water droplets can eject mature sperm some distance from the perigonium, therefore closer to the female gametophore, and shorten the swimming distance for the sperm to reach the egg.

- apical cell is not used up and new growth occurs at the apex..may see a series of perigonial gamtophores like beads on a string.

 

- The female gametophores, when mature are terminated by perichaetia formed of perichaetial leaves enclosing paraphyses and archegonia and retain moisture within the capillary spaces along them.

- the perichaetial leaves are narrower and more elongate than the vegetative leaves ( in some species the perichaetial leaves do not have lamellae)

- the perichaetium, however, exhausts the apical cell in its production, therefore any further apical growth of this gametophore is through the production of a new apical cell below the perichaetium; this innovation allows elongation of the stem via a lateral bud (innovations)

 

            The erect male and female gametophores often form dense turfs with the rhizoids firmly intertwined among gametophores and with the soil.

 

Calyptra is hairy (hairs point downward)

 

How do the Polytrichums prevent water stress?

- cuticular resistance

- internal replenishment of water

- a drop in the ratio between  carbon dioxide uptake and boundary layer resistances afforded by mesophyll-like tissues

- turgor-driven leaf folding

 

Sporophyte:

            The sporophyte in the hair-cap mosses, like most mosses, consists of a foot embedded in the gametophore, and a seta terminated by the sporangium.  The foot possesses transfer tissue that allows for translocation of water (and its dissolved minerals), plus metabolites produced by the gametophore, to the sporophyte.

 

- the whole sporophyte, when immature, is an effective photosynthetic organ, gaining its water, minerals and much of its metabolite from the gametophore.  It is unable to absorb water directly, therefore depends on the gametophore. 

 

 

(a) seta

- The length of seta and sporangium size show similar diversity, but rarely does the seta exceed 10 cm and the sporangium is usually less than 5 mm long.

Fig 6-9

- in cross-section, consists of highly cutinized epidermal cells enclosing a layer of stereid cells and internal parenchyma. 

- a central strand of hydroids encircled by a layer of leptoids complete the anatomy of the seta. 

- the internal conducting system allows for more efficient transfer to the top of the sporophyte, and to the elongation of the sporophyte preceding its differentiation of the terminal sporangium. 

 

(b) sporangium

- The calyptra is often covered in hairs (branched, uniseriate hairs), hence the name, hair-cap mosses. (note an exception Atrichum)

Hand-out

- stomata often on sporangial wall, often in grooves between the apophysis and the rest of the sporangium,  sometimes the guard cells may not be completely separated (therefore the stoma is a single cell with a slit in the middle). These enhance gas exchange in the young sporophyte and undoubtedly are important in the upward movement of water and metabolites from the gametophore to the sporophyte.

- the sporangium, when mature, has an operculum

 

Polytrichaceae

- within the sporangium the cylindric columella, usually expanded at its apex beneath the operculum, forms the epiphragm, and is surrounded by the cylinder of sporogenous cells.

- air chamber with filaments for support

- the peristome teeth that ring the internal margin of the sporangium mouth, overarch the epiphragm, and are attached to its surface. 

- this results in tiny holes between the teeth and their attachment to the epiphragm. 

- the calyptra falls and exposes the operculum. When the sporangium dries, the operculum falls off and exposes the peristome teeth and epiphragm.  Any pressure on the epiphragm or sporangium wall, or any jarring of the sporangium, puffs the spores through these tiny holes, and disperses them into the air.

 

- amphithecial in origin, the two inner layers undergo some periclinal divisions to produce a layer about 4 cells thick

- the teeth are made up of uniformly thickened cells

- the cells at the base of the tooth are more or less isodiametric, while they gradual become more elongate as you go up.

 - in the Polytrichaceae there are generally 32-64 solid teeth (rarely 16)

 

- some species have no peristome (Alophosia, Bartramiopsis, Lyelia)

 

Dawsoniaceae

- NewZealand and Polynesian islands

- sporangium is lunate in cross-section

- in Dawsonia (Dawsoniaceae) the peristome is made up of elongated thick-walled cells which remain separate, or united only in small groups, thus resulting in the shaving brush" appearance

- many long, multicellular filaments formed by asymmetric cell divisions in about 8 concentric layers of cells in radial rows (inner amphithecium)

- no epiphragm

 - there is some controversy about the homology between these two types of teeth, but it is generally accepted that at least the cells at the base of the teeth are homologous.  The endothecium of the Dawsonia may be incorported into the teeth (Edwards doesn’t think so), whereas the teeth of the Polytrichaceae is of amphithecial origin. (abscission layer of the inner side of the teeth in P. commune is the innermost amphithecium.

 

- interesting that taxonomy of the bryophytes is based on sporophytic material.  These are so different, but gametophytically very similar!

Should this be reflected taxonomically?????